Tracking sub-microscopic protein organisation at the plasma membrane of live cells using triple-colour super- resolution microscopy
نویسنده
چکیده
Cell signalling is regulated by submicroscopic features of the plasma membrane, including the actin cytoskeleton and clathrin-coated pits. Total internal reflection microscopy is ideal for studying this, but resolution is limited to approximately 200 nm. This article explains how, at the University of Osnabrück, we have established a triple-colour super-resolution imaging approach to attain an average resolution of just 25 nm. Combining direct stochastic optical reconstruction microscopy (dSTORM) with fluorescence photoactivation localisation microscopy (FPALM), we revealed rapid nanoscale organisation and dynamics of both the cytoskeleton and two cell surface receptor subunits in vivo.
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